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Table 1 Primers used for quantitative RT-PCR on hCMEC/D3 cells

From: Cytokine-induced changes in the gene expression profile of a human cerebral microvascular endothelial cell-line, hCMEC/D3

Category

Gene

Forward and reverse primers

Gene entrez

Start

Stop

Cell-cell contact

CDH5

F: 5′CAGATCTCCGCAATAGACAAGG3′

NM_001795.2

1563

1585

R: 5′CGTGATTATCCGTGAGGGTAAAG3′

1637

1660

MARVELD2

F: 5′GTACTCGTGGTTGCTGGATTAG3′

NM_001038603.2

840

862

R: 5′GCCACCAATTAGAGTCCAGAAG3′

921

943

ANXA2

F: 5′GAAACAGCCATCAAGACCAAAG3′

NM_001002858.2

254

276

R: 5′TGGTAGGCGAAGGCAATATC3′

335

355

Cell-to-matrix adhesion

ITGB1

F: 5′CATGTTGTGGAGAATCCAGAGT3′

NM_002211.3

2367

2389

R: 5′GCAGTAATGCAAGGCCAATAAG3′

2445

2467

ELMO1

F: 5′GACCTGGGTTGAGATGATATGG3′

NM_014800.10

4407

4429

R: 5′GAGTGCTCTGATGGGAAGAAG3′

4483

4504

FHL2

F: 5′CAGAAACTCACTGGTGGACAA3′

NM_201555.1

792

813

R: 5′ATTCCTGGCACTTGGATGAG3′

870

890

Chemokines/cell adhesion molecules

CCL2

F: 5′GGCTGAGACTAACCCAGAAAC3′

NM_002982.3

13

34

R: 5′GAATGAAGGTGGCTGCTATGA3′

108

129

VCAM-1

F: 5′CATTTGACAGGCTGGAGATAGA3′

M73255.1

1217

1239

R: 5′CTCTTGGTTTCCAGGGACTT3′

1297

1317

CEACAM1

F: 5′CTACCTGTAGGATCAGGGTCTAA3′

NM_001712.4

1991

2014

R: 5′CTAGTTGCTTCTAGTGGGTTCTC3′

2069

2092

Transporters

ABCB1

F: 5′TGCAGGTACCATACAGAAACTC3′

NM_000927.4

3264

3286

R: 5′ACCGGAAACATCCAGCATAG3′

3349

3369

SLC2A1

F: 5′GGACAGGCTCAAAGAGGTTATG3′

NM_006516.2

3217

3239

R: 5′AGGAGGTGGGTGGAGTTAAT 3′

3309

3329

SLC2A3

F: 5′CTTAGTTCTCACTGTTCCCTCTG3′

NM_006931.2

3397

3420

R: 5′TCCCAAAGTGCTGGGATTAC3′

3480

3500

Internal standard

ACTB

F: 5′GGACCTGACTGACTACCTCAT 3′

NM_001101.3

633

654

R: 5′CGTAGCACAGCTTCTCCTTAAT 3′

718

740