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Figure 1 | Fluids and Barriers of the CNS

Figure 1

From: Cytokine-induced changes in the gene expression profile of a human cerebral microvascular endothelial cell-line, hCMEC/D3

Figure 1

Validation of mRNA microarray findings by quantitative RT-PCR. Levels of 12 genes selected for validation were determined by quantitative RT-PCR (qRT-PCR). qRT-PCR data parallel that from the mRNA microarray analysis for genes associated with (A) chemokines/CAMs, (B) cell-cell contacts, (C) cell-matrix adhesion, and (D) transporters. Control values were normalized to one and results are expressed as levels in cytokine-treated cells relative to those in unstimulated cells. Actin-β was used as an internal standard. *P < 0.05, **P < 0.01, ***P < 0.001 compared to unstimulated cells. Note that for ELMO and MARVELD2 the signal intensity in cytokine-treated cells was detected at the background level in the mRNA microarray analysis.

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Fluids and Barriers of the CNS

ISSN: 2045-8118