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Table 11 What QTAP can evaluate in an in vitro study

From: A study protocol for quantitative targeted absolute proteomics (QTAP) by LC-MS/MS: application for inter-strain differences in protein expression levels of transporters, receptors, claudin-5, and marker proteins at the blood–brain barrier in ddY, FVB, and C57BL/6J mice

1. Protein abundance in the plasma membrane and/or organelles, including the cytoplasm.
2. Differential protein expression in the organ from which the cells originated.
3. Differential protein expression in the different sides of membrane vesicles.
4. Differential protein expression of cellular characteristics under various cell culture conditions such as co-culture, conditioned medium and passage number.
5. Alteration of cellular characteristics at various stages of differentiation.
6. Expression level of target protein(s) over-expressed in cells by transfection of the corresponding gene.
7. Expression level of an endogenous protein(s) to select a host cell line for the transfection of an exogenous gene and to validate the up-regulation and/or down-regulation of functionally related proteins in the cells after transfection of an exogenous gene.
8. Expression level of target proteins generated by an artificially transfected exogenous chromosome, such as one from a different species of animal, in the cells.
9. Cellular characterization of xenograft tissues before and after their transplantation into in vivo animals.