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Table 10 What we did with quantitative targeted absolute proteomics (QTAP)

From: A study protocol for quantitative targeted absolute proteomics (QTAP) by LC-MS/MS: application for inter-strain differences in protein expression levels of transporters, receptors, claudin-5, and marker proteins at the blood–brain barrier in ddY, FVB, and C57BL/6J mice

1. Protein quantification.
2. Simultaneous quantification of hundreds of proteins in a single analysis.
3. Peptide with a maximal sensitivity of 20 attomole/injection, which corresponds to that of antibody detection.
4. Establishment of a quantitative assay based on only the protein sequence database; the standard protein does not need to be used to select target peptides.
5. Optimization of the peptide sequence produced by various proteases in silico.
6. Differentiation between a peptide and a peptide with a single amino acid substitution.
7. Differentiation between a peptide and its chemically modified form.
8. Virtual SRM/MRM-based detection of hundreds of proteins in a single preliminary assay.