Table 10 What we did with quantitative targeted absolute proteomics (QTAP)

1.

Protein quantification.

2.

Simultaneous quantification of hundreds of proteins in a single analysis.

3.

Peptide with a maximal sensitivity of 20 attomole/injection, which corresponds to that of antibody detection.

4.

Establishment of a quantitative assay based on only the protein sequence database; the standard protein does not need to be used to select target peptides.

5.

Optimization of the peptide sequence produced by various proteases in silico.

6.

Differentiation between a peptide and a peptide with a single amino acid substitution.

7.

Differentiation between a peptide and its chemically modified form.

8.

Virtual SRM/MRM-based detection of hundreds of proteins in a single preliminary assay.