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Figure 5 | Fluids and Barriers of the CNS

Figure 5

From: A study protocol for quantitative targeted absolute proteomics (QTAP) by LC-MS/MS: application for inter-strain differences in protein expression levels of transporters, receptors, claudin-5, and marker proteins at the blood–brain barrier in ddY, FVB, and C57BL/6J mice

Figure 5

Typical calibration curve for absolute quantification. Dilution series of the St peptides (5, 10, 50, 100, 500 and 1000 fmol) and 500 fmol IS peptides were subjected to LC-MS/MS and analyzed in SRM/MRM mode under optimized analytical conditions. The calibration curves were prepared using every transition set by plotting the peak area ratios of the St and IS peptides (y-values) against the St peptide amounts (x-values). This figure represents the calibration curve for mdr1a. R2, correlation coefficient.

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