Figure 4

Schematic representation of BMEC-NPC co-culture schemes. a) NPCs were first utilized to examine non-contact interactions with rat BMECs. b) NPCs of rat and human origin were pre-differentiated to mixtures of neurons, astrocytes, and oligodendrocytes and co-cultured with rat BMECs. Human NPCs differentiated for 9 days yield progeny such as βIII tubulin⁺ neurons (left panel; red) and GFAP⁺ astrocytes (right panel; red) with extensive nestin expression (green). Scale bars indicate 50 μm.