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Figure 2 | Fluids and Barriers of the CNS

Figure 2

From: Modeling the blood–brain barrier using stem cell sources

Figure 2

Schematic representation of the various BBB in vitro models. Cells are isolated from whole brain tissue (non-human origin) or from biopsied tissue samples (human origin). From these sources, primary cultures of BMECs, astrocytes, pericytes and neurons can be achieved. In the case of BMECs, immortalized cells lines have been established from both rodent (bEnd.3, RBE4) and human (hCMEC/D3) cells. Cells can be cultivated in either a BMEC monoculture or in a co-culture model including any combination of astrocytes, pericytes and neurons. Co-cultures can be established in a non-contact manner or in a contact manner by seeding a co-cultured cell on the other side of the filter.

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