Table 1 Published immortalized human brain EC lines
From: The hCMEC/D3 cell line as a model of the human blood brain barrier
Cell lines | Immortalization procedure | Phenotype/limitations | TEER (Ω.cm2) | LY/sucrose permeability coefficient (10-3cm/min) | Year (first pub) | Ref. |
|---|---|---|---|---|---|---|
BB19 | HPV16 E6E7 | Constitutive expression of VCAM-1, CD36 | nd | 1.35 | 1996 | |
HCEC | SV40T | No permeability characterization | 40±8 | nd | 2000 | [70] |
HBEC-5i | SV40T | No expression of CD31 | 180±10 | nd | 2006 | [71] |
NKIM-6 | HPV16 E6E7 | No expression of claudin-5 or occludin | 100 | nd | 2007 | [72] |
HBMEC-3 | SV40T | No information available | 245±8 | nd | 2009 | [73] |
TY08 | Temperature-sensitive SV40T | Low level of functional P-gp | 37±5 | Pe(inulin)=1.23 | 2010 | [74] |
HBMEC/ciβ | Temperature-sensitive SV40T and hTERT | Promising preliminary characterization | nd | 2.6±0.4 | 2012 | [75] |