Figure 5
From: Organotypic brain slices: a model to study the neurovascular unit micro-environment in epilepsies
Organotypic slices are accessible for drugs testing. (A) Immunostaining of ZO-1 (green) and laminin (red) in control slices and 24 h post-kainate seizures with or without rh-Ang1 application 4 h post-seizures. Rh-Ang-1 repaired the loss of ZO-1 (green) in blood vessels (laminin, red). Scale bar: 50 μm (B) Analysis of Zonula occludens-1 expression by Western blot 24 h post-kainate treatment with or without rhAng1 application. Results are expressed in percent of control, * p<0.05.