Figure 4

Organotypic slices are accessible for analysis of proteins in tissue. (A) Analysis of VEGF expression by Western blot 2, 12 and 24 h post-bicuculline treatment. Results are expressed in percent of control, * p<0.05. (B) Immunostaining of VEGF (green), NeuN or laminin (red) and GFAP (blue) in control slices or 12 h post-bicuculline seizures (PS). VEGF is expressed in neurons and in astrocytes around blood vessels (arrows), Scale bar 10 μm. (C) Analysis of VEGFR-2 expression and activation by Western blot 2, 12 and 24 h post-bicuculline treatment. Results are expressed in percent of control, * p<0.05. (D) Immunostaining of VEGFR-2 (green), NeuN or laminin (red) in control slices or 12 h post-bicuculline seizures. VEGFR-2 is expressed in neurons and along blood vessels 12 h after bicuculline treatment. Scale bar 10 μm. (E) Analysis of Ang1 and Ang2 expression by Western blot 24 h post-bicuculline or kainate treatment. Results are expressed in percent of control, * p<0.05, ** p<0.01.