Figure 3
From: Culture models to study leukocyte trafficking across the choroid plexus
3-D Analysis of PMN transmigration across PCPEC. Three-dimensional immunofluorescence images of stimulated PCPEC co-cultured with PMN were reconstructed from 0.3 μm Apotome optical sections, using Zeiss software Inside 4D. A-C. S. suis stimulated PCPEC. Views from above on the apical cell side (A,B) show transcellular PMN migration, in which the centrally localized PMN migrates distal to the TJ. The sideview indicated that the PMN is about to leave the cell (C). D-E. TNFα-stimulated PCPEC. A centrally localized PMN (A,B), that transmigrates from the basolateral to the apical direction (C) is demonstrated. This figure is a representative example of three independent experiments that all gave similar results. Scale bar, as indicated.