Figure 2
From: Culture models to study leukocyte trafficking across the choroid plexus
Transmigration of PMN across PCPEC after stimulation with S. suis or TNFα. En face Apotome microscopy of monolayers stained for occludin (red), actin (phalloidin, green) and nuclei (DAPI, blue) after PMN (SWC3a-FITC, yellow) transmigration. Top and side of each panel is a cross section through the z-plane of multiple optical slices. A-C. S. suis stimulated PCPEC. PMN is localized within the cell body, surrounded by tight junctions (red arrows) and actin (white arrows). Another PMN is leaving the cell close to the tight junction (yellow arrows). Moreover tight junction and actin cytoskeleton disarrangement is demonstrated. D-F. TNFα-stimulated PCPEC. Several PMN close to tight junctions and in the middle of PCPEC cell body are demonstrated. One PMN is about to leave the cell via the apical membrane (white arrows). Another PMN seems to transmigrate via the paracellular route, since the occluding morphology is strongly altered in the area the PMN is transmigration through (red arrows). This figure is a representative example of three independent experiments that all gave similar results. Scale bar, 10 μm.