Figure 7
From: Hydrocephalus induces dynamic spatiotemporal regulation of aquaporin-4 expression in the rat brain
Double immunofluorescence for aquaporin-4 and glial fibrillary acidic protein in control and two week hydrocephalic rat. Aquaporin-4 is visualized with TxRED (red) and glial fibrillary acidic protein with FITC (green). Nuclear counterstaining with DAPI (blue). Pictures represent glia limitans (A-F), periventricular region (including ependyma) (G-L) and vessels (M-R). Yellow colours in merged pictures confirm co-localization of aquaporins-4 and astrocytic GFAP in glia limitans and in perivascular endfeet. Both in control and in all hydrocephalic groups aquaporin-4 and glial fibrillary acidic protein showed co-localisation in the astroglial endfeet surrounding the vessels (M-R) and in glia limitans (A-F). Astrocytic cell soma were mainly without evidence of aquaporin-4 expression. * = lateral ventricle. Scale bar 100 μm.