Figure 6

Aquaporin-4 immunohistochemistry of the ependyma. The aquaporin-4 positive ependymal cell lining of each rat was categorized as belonging to one of four different aquaporin-4 immunoreactivity morphologies (single-layered cuboidal (A), multilayered cuboidal (B), no staining (C) and flattened ependyma (D)). The table shows the proportion of animals in each group belonging to each morphological category. Cuboidal ependyma in single- or multilayered patterns were found in all groups. In the two day group, two cases were found to have lost ependymal staining. At two weeks a diversity of morphologies were reported ranging from single layered cuboidal to flattened in the case of most extreme hydrocephalus. No clear evidence of apical expression of aquaporin-4 in ependymal cells was found at any time point. Scale bar 50 μm.