Table 1 Primer sequences and the expected size of the PCR products.
From: Expression and functional activity of nucleoside transporters in human choroid plexus
Gene of interest | NCBI Accession number | Position in the mRNA | Amplicon length | Amplicon sequence | Probe sequence |
|---|---|---|---|---|---|
hENT1 | NM_001078174 | 358-433 | 75 | ACAGATACAAAGCGTCT GGCTT ATCTTCTTCATGCTG GGTCTGGGAACGCTGCTC CCGTGGAATTTTTTCATGA | CAGCCTCAGGACAGATACAAAGCTG |
hENT2 | NM_001532 | 1290-1400 | 110 | CAGACGAGGACAGCCGG CTGCTGCCCCTGCTGGTCTGCCTGCGGTTCCTGTTCGTGCCCCTCTTCATGCTGTGCCACGTGCCCCAGAGGTCCC GGCTGCCCATCCTCTTCC | CTTACTTCCTGTGGCCAGACGAGGA |
hENT3 | NM_018344 | 827 - 909 | 82 | CCAGGTACTACATGAGGCCTGTTCTTGCGGCCCATGTGTTTTCTGGTGAAGAGGAGCTTCCCCAGGACTCCCTCAGTGCCCC | GGAGTATGCCAGGTACTACATGAGG |
hCNT1 | NM_201651 | 391 - 463 | 72 | GAACCTGCAGCCAGCCCTGA GAGCCAGAAGCTTCTG CAGGGAGCACATGCAGCTGTTTCGATGGATCGGCAC | TCCAGATGGAGGAACCTGCAGCCAG |
hCNT2 | NM_004212 | 236 - 301 | 65 | TCGGTGGCCTTTCAGCAAAGCAAGAAGTTTCTGCAAAACACACGCCAGATTGTTCAAGAAGATCC | ACTTACCAGAGGAGGAGTCGGTGGC |
hCNT3 | NM_022127 | 617-718 | 101 | GGTG ATCTGGAGCTCCCT GGTCCT AGCAGTTATTTTCTG GTTGGCCTTTGACACTGCCAAATTGGGTCAACAGCAGCTGGTGTCCTTCGGTGGG CTCATAA | CTGGCTGAAGTGGGTGATCTGGAGC |
18 S | X03205 | 609-796 | 187 | G GTGCCAGCAGCCGCG GTAATTCCAGCTCCAATAGCGTATA TTAAAGTTGCTGCA GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGA GCCACCGCCCGTCC CCGCCC CTTGCCTCTCGG CGCCCCCTCGATGCTCTTAGCTGAGTGTCCCGCGGGGC CCGAAG | ACTTGCTCTTGGACAGGAACCAGGG |