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Figure 3 | Cerebrospinal Fluid Research

Figure 3

From: Development of the lateral ventricular choroid plexus in a marsupial, Monodelphis domestica

Figure 3

Fluorescent double labelling of endogenous plasma protein and BrdU. A. P3 Monodelphis 2 sh after a single injection of BrdU. Nuclei positive for the cell division marker BrdU are visible (unfilled arrows, red nuclei), as well as choroid plexus epithelial cells positive for plasma protein (filled arrow, green cells). Nuclei were seen only to appear from the root on the upper (dorsal) side of the plexus. There were no positive nuclei further along the plexus than protein positive cells. No plexus cells positive for protein were double-labelled for BrdU at this age. B. P10 Monodelphis injected with BrdU at P3, P4 and P5. BrdU positive nuclei (unfilled arrows, red nuclei) were seen further along the stalk of the plexus. Some cells were positive for plasma protein (green) and double labelled with BrdU (filled arrow). BrdU positive nuclei were only seen on one side of the plexus. No positive nuclei were seen in the root on the contralateral side (asterisk). C. Higher power image of double-labelled cell in B. Abbreviations: cp, choroid plexus; lv, lateral ventricle; ne, neuroepithelium. Scale bar: 25 μm in A, C; 50 μm in B.

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