Figure 8
From: Aquaporins: relevance to cerebrospinal fluid physiology and therapeutic potential in hydrocephalus
Lentiviral-GFP localisation in choroid plexus epithelial cells in mice. Normal Quackenbush Swiss mice received intraventricular injection of lentiviral-GFP (107 virus genomes in 10 μL of sterile saline) and harvested after 7 days of injection. Frozen coronal section of the brain were incubated with rabbit GFP antibody at 4°C overnight followed by incubation of anti-rabbit Ig conjugated with Alexa 488. GFP signals were found in the cytoplasm of some choroid epithelial cells (arrows) in lateral choroid plexus in panel A (confocal image) and C (composite of A and B). Panel B is the phase contrast image of the same field in A. (Owler et al., unpublished data).