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Figure 8 | Cerebrospinal Fluid Research

Figure 8

From: Co-localization and regulation of basic fibroblast growth factor and arginine vasopressin in neuroendocrine cells of the rat and human brain

Figure 8

Immunoblotting confirms enhanced hypothalamic FGF2 expression after dehydration: FGF2 protein level was analyzed by western blotting for control (C) and dehydrated (D) states. Note the increased FGF2 levels in hypothalamus, resulting from 3 days of water deprivation, for the 3 isoforms of about 18, 23 and 24 kDa. Heart, kidney and brain rat tissues were extracted as described in the text and examined for rat FGF2 with a polyclonal antibody recognizing 3 forms of endogenous FGF2. Equal amounts of protein were loaded per well. Exposure time was 36 h. Dehydration specifically elevated the expression of FGF2 in hypothalamus but not in heart or kidney.

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