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Table 1 RNA expression of TJ proteins occludin, claudin 1, 2 and 11

From: Characterization of immortalized choroid plexus epithelial cell lines for studies of transport processes across the blood-cerebrospinal fluid barrier

   TR-CSFB Z310 primary cells CP
   values shown as 2ΔCqGAPDH- ΔCqGOI× 103
   Average SE Average SE Average SE Average SE
claudin1 untreated 0.017 0.006 0.017 0.003 13.024 2.220 67.913 1.077
claudin1 100nM HC 0.346 0.070 2.521 0.121     
claudin1 550nM HC 0.478 0.053 3.743 0.422     
claudin1 1 μM Dex 2.610 0.124 1.424 0.135     
claudin2 untreated 1.907 0.699 0.203 0.090 3.566 0.329 364.891 59.225
claudin2 100nM HC 0.417 0.161 3.976 0.068     
claudin2 550nM HC 0.450 0.186 6.635 0.661     
claudin2 1 μM Dex 4.686 1.715 0.965 0.302     
claudin11 untreated 0.326 0.066 0.051 0.012 5.112 2.717 3.215 0.682
claudin11 100nM HC 0.042 0.014 0.103 0.019     
claudin11 550nM HC 0.067 0.030 0.119 0.025     
claudin11 1 μM Dex 0.427 0.280 0.054 0.009     
occludin untreated 0.130 0.067 3.002 1.120 15.652 5.865 72.499 6.147
occludin 100nM HC 6.580 1.181 34.882 3.896     
occludin 550nM HC 4.943 1.237 37.174 4.639     
occludin 1 μM Dex 7.899 1.286 17.922 4.243     
  1. mRNA expression of the CP TJ proteins claudin1, 2, 11 and occludin in TR-CSFB and Z310 cells comparing untreated cells with 100nM, 550nM hydrocortisone and 1 μM dexamethasone treated cells as well as with primary cells and CP tissue. Cq values were normalized to the reference gene GAPDH according to 2ΔCqGAPDH-ΔCqGOI and multiplied with 103 for better readable format (values in table). Bold values indicate high or elevated RNA expression. Claudin1 and claudin2 were highly up-regulated in TR-CSFB cells after treatment with dexamethasone, while hydrocortisone treatment had a higher impact on claudin1 and 2 RNA expression in Z310 cells. Occludin on the other hand was up-regulated by all corticosteroid treatments, but again dexamethasone had a higher impact on its expression in TR-CSFB, while hydrocortisone elevated occludin expression in Z310 twice as much as dexamethasone.