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Figure 4 | Cerebrospinal Fluid Research

Figure 4

From: Characterization of immortalized choroid plexus epithelial cell lines for studies of transport processes across the blood-cerebrospinal fluid barrier

Figure 4

Western blots with anti-occludin antibodies and anti-claudin-1 show higher expression of the two TJ proteins in Z310 compared to TR-CSFB cells and slightly increasing expression after treatment with glucocorticoids, dexamethasone (Dexa) and hydrocortisone (HC). 20 μg of each protein were applied. Occludin shows 2 bands, representing the phosphorylated momomeric subunit at 65kD and the unphosphorylated subunit at 51 kD. After detection of occludin and claudin-1, blots were stripped and Na/K-ATPase was detected.

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