Figure 5

Photomicrographs representing RAGE immunohistochemistry of a positive control (adult control animal that is positively stained with RAGE; A), 21-day saline controls (B, C, D), and age-matched hydrocephalic animals (E, F, G). Arrows represent blood vessels that are positively stained with RAGE proteins, characterized by either dark brown punctate particles (A, C, D, G) or diffused profiles (B, E). The asterisks represent non-specific staining of neuron and glial cell bodies. At higher magnification (inset), positive RAGE labeling can be seen within the endothelial lining (arrow) of a capillary deep within the hippocampus. In the choroid plexus of 21-day controls (B), RAGE labeling appears throughout the cytoplasm of epithelial cells but seems more intense on the apical surface adjacent to CSF. In 21-day hydrocephalic animals (E), RAGE labeling is also present throughout the cell but seems to have translocated to the nucleus. Insets show these features at higher magnification. There are minimal differences between the hydrocephalic animals (F, G) versus the saline controls (C, D), i.e. only a slight decrease in the cortex and no change in the hippocampus. In C, D and G, the insets represent a higher magnification to show the punctate character of some RAGE labeling. Scale bar = 25 μm for all panels.