Figure 3

Photomicrographs representing amyloid beta immunohistochemistry of an adult control animal that is positively stained with Aβ antibody(positive control; A, E), 21-day saline controls (B, C, D), and age-matched hydrocephalic animals (F, G, H). In the adult tissue at higher magnification (A, E), positive Aβ labeling can be seen as punctate or more diffuse profiles within and adjacent to the walls of capillaries. In the choroid plexus of neonatal animals, positive Aβ staining, characterized by dark brown punctate particles (arrows) in individual cells, was only seen in hydrocephalic animals. In the cortex and hippocampus, no Aβ labeling was found in either 21-day hydrocephalic animals (G, H) or age-matched controls (C, D). In A, E, and F, the inset represents a higher magnification to show the punctate character of some Aβ labeling. Scale bar = 25 μm.