Figure 4

Ciliary beat frequency analysis in A _2B ^-/-/β-gal reporter gene knock-in mice. (A) Histogram showing the absence of ciliary beat frequency enhancement due to 1 μM NECA (n = 10) and 30 μM adenosine (n = 10) in the A_2B^-/- mice. (B) Enhancement due to 300 μM BzATP application was reduced in the A_2B^-/- mice (n = 8) versus wild-type P2X₇^+/+ mice (n = 5). BzATP-induced enhancement was eliminated in the A_2B^-/- mice after pre-incubation of the slices with 100 nM BBG (n = 8). *: P < 0.05 for all panels, data are means ± SEM. The n value indicates the number of slices tested. (C) Summary diagram showing enzymatic breakdown of BzATP and subsequent receptor activation. ATP (an endogenous signaling molecule analogous to BzATP) is shown in grey.