Figure 9

Lipid liquid chromatography mass spectrometry data, representative of four analyses from separate CSF samples. Unique lipid components differentiate P3 and S3 preparations. Total ion profiles (TIC) for A: S3, and B: P3 show the elution profile from the normal phase column for lipids: PG/PE: phosphatidylglycerol/phosphatidylethanolamine and PC: phosphatidylcholine were decreased in P3 fractions. CS: cerebroside; CM: ceramide; PI: phosphatidylinositide; PS: phosphatidylserine; PA: phosphatidic acid; SPM: sphingomyelin; PAF: platelet-activating factor; LPC: lysophosphatidylcholine. C and D: The inositol polyunsaturated fatty acid-containing lipids are greatly increased (Peak 1) in the P3 fractions. The omega 3 polyunsaturated fatty acids are increased in P3 fraction, E and F: EPA: eicosanpentanoeate, G and H, and DHA: docosahexaenoate J and K. The omega-6 polyunsaturated fatty acid is decreased, AA: arachidonic acid. The ratio of omega-3/omega-6 is significantly higher in P3 fractions (p = 0.0005).