Figure 10
Prostanoid regulation in CSF. A: prostaglandin H synthase (PGHS-1 and PGHS-2) activity assays for P3, S3, and S1 from two different study participants, analyzed for a total of at least three measures per fraction, with standard error bars. Both PGHS-1 & PGHS-2 specific activities are demonstrated in P3 and S1 versus S3 fractions, compared to baseline activities without inhibitor. This demonstrates the presence of specific PGHS-1 and -2 activities in the S1 fractions that are enriched in the P3 (and decreased in the S3) fractions. B: Scheme for prostanoid enzymes, receptors, and regulators identified in CSF by shotgun liquid chromatography mass spectrometry (blue) and substrates identified by LCMS in SRM mode (green). Prostaglandins were not identified in this study (black). This diagram outlines CSF components capable of extensive prostanoid synthesis, with receptors and regulators, including the functional enzymes PGHS-1 and -2 (Figure 10A), the critical source of prostaglandin H2 (PGH2). PLA2: phospholipase A2; PTGDS: prostaglandin D synthase; PGES: prostaglandin E synthase; PGIS: prostaglandin I synthase; THAS: thromboxin A synthase; PGD: prostaglandin D; PGE: prostaglandin E; PGI: prostaglandin I; TXA: thromboxane A; PD2R: prostaglandin D2 receptor; PE2R1, 2, 3, 4: prostaglandin E1, 2, 3, 4 receptors; PI2R: prostaglandin I2 receptor; TA2R: thromboxane A2 receptor; FEM1A: Prostaglandin E receptor 4-associated protein.