The subcommissural organ of the rat secretes Reissner's fiber glycoproteins and CSF-soluble proteins reaching the internal and external CSF compartments

Table 1 Immunoreactivity to AFRU and lectin binding of proteins extracted from the bovine, rat and mouse subcommissural organ

MW	*Bovine SCO (adult)			Rat SCO (PN30)			Mouse SCO (PN30)
	AFRU	Con A	WGA	AFRU	Con A	WGA	AFRU	Con A	WGA
630				+	+	-	+	+	-
540	+	+	-
450	+	+	+	+	+	+	+	+	+
390	+	+		+	+	-	+	+	-
320	+	+	-	+	+	+	+	+	+
200				+	+	+

MW, molecular weights in kilo Daltons; SCO, subcommissural organ; AFRU, antibody against all glycoproteins of bovine RF; Con A, concanavalin A (affinity = mannose, glucose); WGA, wheat germ agglutinin (affinity = glucosamine, sialic acid); +, positive reaction; -, negative reaction. *From Nualart et al. 1991 [12].

ISSN: 2045-8118