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Table 1 Immunoreactivity to AFRU and lectin binding of proteins extracted from the bovine, rat and mouse subcommissural organ

From: The subcommissural organ of the rat secretes Reissner's fiber glycoproteins and CSF-soluble proteins reaching the internal and external CSF compartments

MW *Bovine SCO (adult) Rat SCO (PN30) Mouse SCO (PN30)
  AFRU Con A WGA AFRU Con A WGA AFRU Con A WGA
630     + + - + + -
540 + + -       
450 + + + + + + + + +
390 + +   + + - + + -
320 + + - + + + + + +
200     + + +    
  1. MW, molecular weights in kilo Daltons; SCO, subcommissural organ; AFRU, antibody against all glycoproteins of bovine RF; Con A, concanavalin A (affinity = mannose, glucose); WGA, wheat germ agglutinin (affinity = glucosamine, sialic acid); +, positive reaction; -, negative reaction. *From Nualart et al. 1991 [12].