Immunoblots of SCO extracts of bovine (adult, lanes 1–3), rat (PN30, lanes 4–6) and mouse (PN30, lanes 7–9). Lanes 1, 4, 7: immunoblots using AFRU. Lanes 2, 5, 8: concanavalin A (Con A) binding. Lanes 6, 9: wheat germ agglutinin (WGA) binding. Lane 3: concanavalin A blocked with mannose prior to binding. No binding is detected (asterisk). Numbers: molecular weight in kDa. Dotted lines: compounds not binding WGA.