Figure 6

Effect of Fgf2 on the expression of genes associated with CPe development and function in TRCSFB-2 cells. The bar charts show the average level of mRNA expression for A: E2f5 (n ≥ 9), B: FoxJ1 (n ≥ 12), C: p73 (n ≥ 7) and D: TTR (n ≥ 11) in response to increasing Fgf2 concentrations (0 ng/ml, 10 ng/ml, 100 ng/ml). Expression of each gene was assessed by RT-PCR and normalised against expression of the housekeeping gene, GAPDH. Both E2f5 and FoxJ1 transcripts are significantly reduced by treatment with 100 ng/ml (p < 0.05). Data are means ± SEM.