Figure 5

Fgf2 effect on early CPe vesicle formation. (A-B) Early formation of vesicles in the presence of 0 ng/ml (A) or 10 ng/ml (B) Fgf2 monitored in the same field of view at one, two and four days after plating in Matrigel. Note that on day 1, just before adding Fgf2 to the cells, CPe aggregates in (A) and (B) are similar in size. On day 2, Fgf2-treated CPe cells have formed bigger aggregates that are fewer in number compared to non-treated cells. On day 4, vesicles in the Fgf2-treated culture are larger than in control culture, but fewer in number. (C) Graph showing the average size of aggregates and vesicles in cultures treated without (control) or with 10 ng/ml Fgf2 at different times after seeding according to a multiple linear regression analysis. The fitted lines show that Fgf2-treated aggregates and vesicles become bigger in size over time. The average size difference between control and Fgf2 treated cultures is 462.5 μm² (SEM: 87.25 μm²), and Fgf2-treated aggregates increase every day on average by 492.03 μm² (compared to control aggregates. (D) Bar chart showing the mean number of aggregates and vesicles in cultures treated without or with 10 ng/ml Fgf2. At day 2 and day 4 after seeding, the number of aggregates and vesicles is significantly lower in Fgf2-treated cultures compared to controls (p < 0.05), whereas before the addition of Fgf2 on day 1, the difference is not significant. Data are means ± SEM.