Figure 2
From: Evidence for reduced lymphatic CSF absorption in the H-Tx rat hydrocephalus model
Surface view of the frozen whole head showing distribution of 50 μl of 2% Evan's blue dye injected into the cistern magna of rats post mortem at 10 days of age. Note blue dye in the subarachnoid spaces over the cortex and the skull base in all 3 groups (white arrows). Top row: coronal views at level of lateral ventricles (a, d, g). Note enlarged lateral ventricles in hydrocephalic animal (g). Middle row: Olfactory region in the sagittal plane of SD (b), H-Tx (e) and affected hydrocephalic animals (h). The overlay grid indicates region of olfactory turbinates for semi-quantitative analysis between groups. Note the dye is present in the subarachnoid spaces around the olfactory bulb (OB) in all animals. The blue tracer passed through the cribriform plate (red arrows) into the olfactory turbinates (OT) of the SD (b) and H-Tx (e) pups. Less dye was observed in the olfactory turbinates of hydrocephalic animals (h). Bottom row: coronal sections showing the nasal regions, showing less dye in the hydrocephalic animal (i). White arrows indicate Evan's blue in subarachnoid spaces, red arrows identify cribriform plate. Reference scales are provided as a ruler (mm) or as a longitudinal bar.