Figure 4

Photomicrographs showing rat brain immunolabeling for decorin at 3 weeks age. In control rats, the cerebral cortex (A) and white matter (C) exhibit minimal immunoreactivity. In age matched hydrocephalic rats, perineuronal labeling is increased in the cerebral cortex (B) and periglial labeling is increased in the white matter (E). No labeling is seen in hydrocephalic white matter when the primary antibody is omitted (D). Scale bar = 100 μm. The bar graph shows densitometric analysis of immunolabeling (arbitrary units) in cortex layer 1 of control (C) and hydrocephalic (H) rats. The hydrocephalus-related increase is significant only in the 3-week rats (*p = 0.0003 ANOVA, Bonferroni-Dunn; all groups n = 4). Note that significant changes were also present in the glia limitans.