Figure 8

Fluorescent labeling of third ventricle region leading to the cerebral aqueduct. Histologic sections were triple labeled to detect the presence of GFAP (red), ILB4 (green) and DAPI (blue). In both the 5d control (A) and hydrocephalic (B) animals and 12d control (C) and hydrocephalic (D), there were no apparent differences in relative abundance of astrocytes or microglia cells. In the 21d animals, there was a remarkable increase in the abundance of astrocytes present in the hydrocephalic animals (F) when compared to the control animals (E). This dramatic increase in staining abundance was also noted between the control (H) and hydrocephalic (I) 36d animals. Shunting these two groups had the effect of reducing staining intensity and density of the astrocyte cells in the peri-aqueductal area of the untreated hydrocephalic animals. There was an increase in the microglial population in both of the shunted groups on the ventricular surface(G and J). Scale bar = 100 μm.