Figure 7

Fluorescent labeling of peri-ventricular white matter (from the parietal cortex region). Histologic sections were triple labeled to detect the presence of GFAP (red), ILB4 (green) and DAPI labeled nuclei (blue). No dramatic differences in relative abundance of staining were detectable between the 5d control (A) and hydrocephalic (B) animals, or between the 12d control (C) and hydrocephalic animals (D). There was increased abundance of GFAP staining in the peri-ventricular white matter of 21d hydrocephalic (F) animals when compared to their matched controls (E), this trend was also noticed between the 36d (I) hydrocephalic rats and their controls (H). Both 21d (G) and 36d (J) shunted rats had a slight increase in overall abundance of staining over control animals (E and H respectively). In the 36d shunted animals (J) GFAP positively labeled cells appeared to not be in a concentrated band as in the 21d shunted animals (G), but were present in a less dense band of staining, with cells migrating and distributed farther away from the peri-ventricular white matter. Microglia distribution in the 21d shunted animals (G) was slightly increased, while the 36d animals (J) did not show dramatic alterations in microglia. White dashed line demarcates cortical grey matter from peri-ventricular white matter. Scale bar = 100 μm.