Figure 6

Histogram of histologic grading for relative abundance of GFAP labeled cells (cortical layers 1–4). There were significant increases in relative abundance of positively stained astrocytes in hydrocephalic rats at all age groups, similar to results from the Western blot experiments. Shunting reduced the abundance of stained cells in the 21d and in the 36d animals, but at 21d this was not significant. One asterisk indicates significance, p < 0.05, using a Mann-Whitney non-parametric test for two group comparisons or Kruskal-Wallis non-parametric test for three-group comparison (n = 5 for each group, values are means +/- standard deviation).