Table 1 The sequences of primers and the predicted sizes of PCR products.
From: Expression of junctional proteins in choroid plexus epithelial cell lines: a comparative study
Species | Gene | Primer | Sequence | Predicted size of PCR product (base pairs) |
|---|---|---|---|---|
Rat | Occludin | F | 5'-GGAACACATTTATGATGAACAGC-3' | 235 |
| Â | Â | R | 5'-CACGGACAAGGTCAGAGGAA-3' | Â |
| Â | Claudin 1 | F | 5'-AAAGATGTGGATGGCTGTCA-3' | 189 |
| Â | Â | R | 5'-AGGAGGCAGAGGGAGGC-3' | Â |
| Â | Claudin 2 | F | 5'-AGGGTTTCCTTAGGGACAATAA-3' | 196 |
| Â | Â | R | 5'-TAAAGTATCTGGTAGGGTTGCC-3' | Â |
| Â | Claudin 11 | F | 5'-CTCATCCTCCCTGGTTACG-3' | 175 |
| Â | Â | R | 5'-GCAGAATAAGGAGCACCCC-3' | Â |
| Â | E-cadherin | F | 5'-AAGAAGACCAGGACTTTGATTTG-3' | 169 |
| Â | Â | R | 5'-GCTGCCTTCAGGTTTTCATC-3' | Â |
Human | Occludin | F | 5'-AGGAACACATTTATGATGAGCAG-3' | 242 |
| Â | Â | R | 5'-GAAGTCATCCACAGGCGAA-3' | Â |
| Â | Claudin 1 | F | 5'-GAAGATGAGGATGGCTGTCA-3' | 142 |
| Â | Â | R | 5'-AAATTCGTACCTGGCATTGA-3' | Â |
| Â | Claudin 2 | F | 5'-ACCATTCCTTGACGGTGTCTA-3' | 121 |
| Â | Â | R | 5'-GCTGATTTTCCATTACGCCT-3' | Â |
| Â | Claudin 11 | F | 5'-TCATCCTGCCGGGCTAC-3' | 174 |
| Â | Â | R | 5'-GCAGAATGAGCAAAACACCA-3' | Â |
| Â | E-cadherin | F | 5'-CCTGCCAATCCCGATGA-3' | 199 |
| Â | Â | R | 5'-TGCCCCATTCGTTCAAGTA-3' | Â |