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Figure 8 | Cerebrospinal Fluid Research

Figure 8

From: Uneven distribution of nucleoside transporters and intracellular enzymatic degradation prevent transport of intact [14C] adenosine across the sheep choroid plexus epithelium as a monolayer in primary culture

Figure 8

Uptake of [14C] adenosine into primary cultured sheep CPEC. The panel shows the cellular uptake from the apical (upper) chamber as a donor (left side) and from the basolateral (lower) chamber as a donor (right). Values shown represent uptake of adenosine, corrected for tracer trapped in the extracellular space, after 5 or 10 min of incubation in the uptake buffer containing [14C] adenosine and [3H] mannitol in the donor chamber. Data are shown for uptake under control conditions in Na+-containing medium (black bars), using Na+-free uptake buffer (grey bars), or in uptake buffer containing both Na+ and 1 uM NBTI (open bars). All values are presented as mean ± SEM from three to five separate inserts obtained from at least two separate isolations. Statistical significance: n.s., P > 0.05 vs. control; **P < 0.01 vs. control by ANOVA.

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Fluids and Barriers of the CNS

ISSN: 2045-8118