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Figure 4 | Cerebrospinal Fluid Research

Figure 4

From: Characterization of cytoskeletal and junctional proteins expressed by cells cultured from human arachnoid granulation tissue

Figure 4

Flow cytometry analysis of cells cultured from AG tissue for expression of the endothelial marker CD31-FITC. A: A Scatter plot of unlabeled cells showing a gate, R2, which was created to define events that were subsequently analyzed for fluorescence. SSC and FSC are side scattered and forward scattered light respectively. B: Histogram used to determine the autofluorescence of unlabeled cells, with the gate M2 defined as the intrinsic FITC fluorescence. C: A scatter plot of cells labeled with the CD31-FITC antibody. The position of the gate R2 in the scatter plot was unchanged from the control analysis. D: Histogram of the 3,767 events that fell within R2 and were subsequently analyzed for CD31-FITC expression. Almost all events (99.8%) also fell within the gate M2, indicating that cells cultured from AG tissue did not express the endothelial marker CD31-FITC. The position of gate, M2, did not change from control analysis.

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Fluids and Barriers of the CNS

ISSN: 2045-8118