Flow cytometry analysis of cells cultured from AG tissue for expression of the endothelial marker VE-cadherin-PE. A: A scatter plot of unlabeled cells, showing a gate, R1, used to define the events subsequently analyzed for fluorescence. SSC and FSC are side scattered light and forward scattered light respectively. B: A histogram was created to determine the autofluorescence of unlabeled cells. The gate M1 is defined as the intrinsic PE fluorescence of unlabeled cells. C: A scatter plots of cells labeled with the endothelial marker VE-cadherin-PE. The position of the gate R1 was unchanged from the control analysis. D: A histogram of the cells labeled with VE-cadherin-PE that fell within the gate R1 and were analyzed for PE fluorescence. Of the 2,601 events that were analyzed, 2,308 (88.7%) fell within the gate, M1, and were considered negative for VE-cadherin expression. The position of the gate, M1, remained unchanged from the control analysis.