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Table 1 Summary of the immunohistochemical characterization of CVOs in health and EAE

From: The circumventricular organs participate in the immunopathogenesis of experimental autoimmune encephalomyelitis

Molecule Healthy1 EAE2
Microvessels
MECA-32 + on all vessels + on all vessels
PECAM-1 + on all vessels + on all vessels
ICAM-2 + on some vessels + on some vessels
ICAM-1 No staining + on most vessels luminally and abluminally/perivascular
VCAM-1 No staining + on few vessels luminally and abluminally or perivascular no luminal VCAM-1 detected in ME
MAdCAM-1 No staining no staining
E-selectin no staining no staining
P-selectin no staining no staining
Immune cells
CD45 + on few perivascular cells + on many cells within the CVOs
CD3 no staining + on few round cells ca. 4 cells/section in SFO ca. 10 cells/section in AP
CD4 no staining + on few round cells
CD8 no staining + on few round cells
B220 no staining + on few round cells
F4/80 only rarely observed on single perivascular cells* + on many exclusively perivascular cells
Mac-1 (αM-integrin) only rarely observed on single perivascular cells* + on round and branched cells located perivascularly and throughout the CVOs
LFA-1 (αL-integrin) only rarely observed on single perivascular cells* + on round and branched cells located perivascularly and throughout the CVOs
α4-integrin no staining very faint staining on few round cells – not regularly detected
MHC class I only rarely observed on few perivascular cells§ considerable staining throughout the CVOs; negative on endothelium
MHC class II only rarely observed on single perivascular cells* + on many perivascular cells
  1. 1All four CVOs were analysed in a total of 10 healthy mice. Results therefore summarize 10 immunostainings. 2A total of 46 mice has been analyzed. Staining for each marker in each CVO was observed at least 10 times. If not stated differently, stainings were repeatedly observed in all CVOs analysed. *Single immunopositive cells were observed in only 1 or 2 of 10 immunostainings. §Few immunopositive cells were observed in 2 out of 10 immunostainings.