Detection of luminal ICAM-1 and VCAM-1 in the capillaries of the ME during EAE. Mice suffering from EAE were perfused intravascularly with antibodies directed against MECA-32, ICAM-1 or VCAM-1; an irrelevant rat IgG was perfused as control. Bound antibody was detected by immunohistology. Only ICAM-1, not VCAM-1, could be detected on the luminal surface of a subpopulation of capillaries in the ME during EAE. Immunoperoxidase, hematoxylin counterstain. Bar = 100 μm. This result was observed 3 times.