Detection of luminal ICAM-1 and VCAM-1 in the capillaries of the SFO during EAE. Mice suffering from EAE were perfused intravascularly with antibodies directed against MECA-32, ICAM-1 or VCAM-1; an irrelevant rat IgG was perfused as control. Bound antibody was detected by immunohistology. Only a subpopulation of capillaries within the SFO stain positive for luminal ICAM-1 and VCAM-1. Immunoperoxidase, hematoxylin counterstain. Bar = 100 μm. This result was observed 3 times.