Immunohistology of the median eminence (ME) during EAE. MECA-32+ capillaries within the ME stain positive for ICAM-1 and VCAM-1. Positive staining for CD45+ can be detected throughout the ME, staining perivascular cells (examples marked by filled arrow) or parenchymal cells (example marked by open arrow). MHC class I is detectable within the entire ME, with parenchymal localization (closed arrows) as well as vessel associated (open arrow). MHC class II is only present on perivascular cells. Immunoperoxidase, hematoxylin counterstain. Bar = 100 μm. This result was observed in at least 10 individual stainings.