Figure 2

Heat shock (stress) protein expression in human CP: Formalin-fixed, paraffin-embedded specimens (8–10 micrometers thick) were de-paraffinized and rehydrated. Sections were incubated overnight with antibodies against HSP90 (1/500; SPA830) and GRP94 (1/500;SPA850) and stained by the ABC technique (Vectastain Elite ABC peroxidase). Deposition of the brown chromogen (diaminobenzidine) reaction product was either substantially reduced by preabsorption blocking or virtually eliminated by omission of either primary or secondary antibodies. Slides were assessed blindly for staining intensity and distribution [37]. See text for description of localization and interpretation. For each HSP, images are representative of 10 AD specimens and 5 age-matched controls.