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Fig. 2 | Fluids and Barriers of the CNS

Fig. 2

From: Single-cell analysis of mesenchymal cells in permeable neural vasculature reveals novel diverse subpopulations of fibroblasts

Fig. 2

TdTom+ cells are restricted to mesenchymal cell types in the CNS. Representative sections counterstained with markers as indicated, tissue sections are in sagittal plane. A Na/K/ATPase staining of the 4th ventricle CP epithelium and PG endocrine cells. B LEPR staining of the CP epithelium and non-tdTom+ cells in the PG. C Staining with ACTA2 and identification of smooth muscle cells. D Microglial marker IBA1 occupies the same connective tissue compartment as tdTom+ cells in CP, but does not colocalize in 4th ventricle CP or PG. E SOX2, a marker of folliculostellate cells in PG and a putative neural stem cell marker, was totally absent from 4th ventricle CP, and does not co-localize with tdTom+ cells in the PG. F Pan-neural marker TUBB3 is absent from the 4th ventricle CP and does not co-localize with tdTom+ cells in the PG

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Fluids and Barriers of the CNS

ISSN: 2045-8118