Fig. 5

C9-ALS BMEC-like cells restrict neurite length in healthy Motor Neurons A) The Lactate Dehydrogenase Assay (LDH) assay was performed on 2 healthy donors and 3 C9-ALS donors hi-PSCs derived BMEC-like cells. Statistical significance was determined using Student’s unpaired t-test (****p < 0.0001). B) The diagram was created on Biorender.com C) Control Motor Neurons (MNs) were fixed with 4%PFA after 72 h on 30% BMEC-like cells conditioned media treatment. Brightfield images are shown as follows: untreated MNs, control BMEC-like cells media (CTR BMECs) and ALS BMEC-like cells media (ALS BMECs) treated MNs. The scale bar equals 50 µM. At least a total of 5 images were acquired per each condition, with a media of 3 replicates for a total of 3 biological replicates (N = 3, total images per condition = 45). D) Neurite length analysis was performed with ImageJ software. Data are plotted as Mean ± SD. Statistical significance was determined using One-Way ANOVA (****p < 0.0001). N = 5