Fig. 6

Effect of PPAR activation upon the expression of Slc16a1/MCT1 in primary cultured rat CECs. (a-c) qRT-PCR analysis of the gene expression of Slc16a1. (a,b) Cells were treated as indicated with synthetic agonists selective for PPAR α (fenofibrate, feno), PPAR δ (GW501516, GW0742), or PPAR γ (rosiglitazone, rosi) and synthetic antagonists selective for PPAR α (GW6471) or PPAR δ (GSK0660). (c) Cells were treated as indicated with palmitate (PALM) or oleate (OLE) in the presence of L-carnitine (carn). (d) Western-blot analysis of the relative expression of MCT1 in cells treated with 400 µM Oleate or 400 µM Palmitate ± 2 µM GSK0660 (GSK) for 48 h. For quantitative comparison, the signal was normalized upon the signal from Actin. (e) qRT-PCR analysis of CECs cultured with various concentrations of Glucose and β-hydroxybutyrate (BHB) for 48 h. The culture medium contained 0.38% BSA in all conditions including control (except in e). (a-e) Mean ± SD (fold increase to the control). One-way ANOVA with Holm-Sidak’s multiple comparisons test