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Fig. 5 | Fluids and Barriers of the CNS

Fig. 5

From: Oxygen glucose deprivation-pretreated astrocyte-derived exosomes attenuates intracerebral hemorrhage (ICH)-induced BBB disruption through miR-27a-3p /ARHGAP25/Wnt/β-catenin axis

Fig. 5

ADVEs abrogated hemin-induced barrier injury in bEnd.3 cells through transferring miR-27a-3p A. RT-qPCR analysis of miR-27a-3p expression in ADEVs from different groups. B. RT-qPCR analysis of miR-27a-3p expression in BMECs from different groups. C. Schematic illustration presented that OGD-stimulated astrocyte was transfected with Cy3 labeled miR-27a-3p mimic, ADEVs extracted was labeled with PKH67 and incubated with bEnd. 3 cells. D. Confocal images of PKH67- labeled ADEVs, including Cy3-miR-27a-3p, internalized by bEnd.3 cells. E. TEER of monolayer bEnd. 3 cells. Scale bar: 20 μm F. The protein levels of occludin, claudin-5 and claudin-5 in bEnd.3 cells determined by western blot. **p < 0.01 versus indicated group; *p < 0.05 versus indicated group. All data were expressed as mean ± SEM of at least 3 independent experiments

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Fluids and Barriers of the CNS

ISSN: 2045-8118