Cocaine regulates antiretroviral therapy CNS access through pregnane-x receptor-mediated drug transporter and metabolizing enzyme modulation at the blood brain barrier

Table 1 Details regarding antibody name, catalogue number, clone, concentration, and company from which it was obtained are provided for antibodies used in the study. Antibodies were knockout verified, are widely published on, and have been certifiably verified by the vendor

Antibody	Company	Catalogue Number	Clone	Concentration for Immunofluorescence	Concentration for Western Blot
GFAP(AF488)	Invitrogen	53–9892-82	GA5	10 μg/mL
GLUT-1	Invitrogen	PA5-16,793	Poly	1:100
CD71	Invitrogen	14–0719-82	OKT9	5 μg/mL
PECAM(FITC)	Invitrogen	11–0311-82	390	5 μg/mL
VE-cadherin	Invitrogen	14–1449-82	16B1	5 μg/mL
Zo-1	Invitrogen	61–7300	Poly	10 μg/mL	1 μg/mL
Occludin	Invitrogen	71–1500	Poly	4 μg/mL	0.5 μg/mL
Claudin 5	Invitrogen	35–2500	4C3C2	1:25	1:1000
OAT1	Invitrogen	PA5-26,244	Poly	1:100	1:1000
OAT3	Invitrogen	PA5-76,143	Poly	1:100	1:1000
ENT1	Proteintech	11,337–1-AP	Poly	1:100
ENT1	Invitrogen	PA5-116,461	Poly		1:500
OATP1A2	Invitrogen	PA5-42,445	Poly	1:100	1:1000
OATP2A1	Invitrogen	PA5-98,789	Poly	1:200	1:1000
BCRP	Novus	NBP2-22,124	3G8	1:200	1:2000
P-gp	Invitrogen	PA5-61,300	Poly	2 μg/mL
P-gp	Abcam	ab261736	Poly		0.5 μg/mL
MRP1	Abcam	ab24102	MRPm5	1:50	1:200
MRP4	Cell Signaling	12,705	D2Q2O	1:200	1:1000
MRP5	Invitrogen	PA5-18,965	Poly	10 μg/mL
MRP5	Abcam	Ab180724	Poly		1:1000
CYP3A4	Invitrogen	MA5-17,064	3H8	1:200	1:1000
PXR	Invitrogen	PA5-72,551	Poly	1:50	1:1000
CAR	R&D Systems	PP-N4111-00	N4111	10 μg/mL	2 μg/mL
AK1	Invitrogen	PA5-52,297	Poly	1 μg/mL	0.1 μg/mL
AK2	Proteintech	11,014–1-AP	Poly	1:200	1:1000
AK5	Invitrogen	PA5-53,933	Poly	1.2 μg/mL	0.09 μg/mL
AK6	Novus	NBP2-67,116	B1-F4	1:100	1:2000
Β-Actin (HRP)	Cell Signaling	12,262	8H10D10		1:5000
Anti-rabbit (HRP)	Abcam	ab97051	Poly		1:2000
Anti-mouse (HRP)	Abcam	ab97023	Poly		1:2000
Anti-rabbit (AF488)	Invitrogen	A-11008	Poly	1 μg/mL
Anti-mouse (AF488)	Invitrogen	A-11001	Poly	0.67 μg/mL
Anti-goat (AF488)	Invitrogen	A-11078	Poly	1 μg/mL

ISSN: 2045-8118