Table 1 Primer sequences
Gene | Forward primer (5’ – 3’) | Reverse primer (5’ – 3’) |
|---|---|---|
mTBP* | ATGGTGTGCACAGGAGCCAAG | TCATAGCTACTGAACTGCTG |
mYWHAZ* | GCAACGATGTACTGTCTCTTTTGG | GTCCACAATTCCTTTCTTGTCATC |
mZO-1 | GGCATTCCTGCTGGTTACA | AGGACACCAAAGCATGTGAS |
mClaudin-5 | GCCCTGCTCAGAACAGACTA | GGCAGTTTGGTGCCTACTTC |
mIL-1β | GCTGAAAGCTCTCCACCTCA | AGGCCACAGGTATTTTGTCG |
mIL-10 | CTTTAAGGGTTACCTGGGTTGC | ATTAAAGGCATTCTTCACCTGC |
mIL-17 | CAGCGATCATCCCTCAAAGC | GCGCCAAGGGAGTTAAAGAC |
mCCL2 | CAGCAGGTGTCCCAAAGAAG | CATTTGGTTCCGATCCAGGTT |
mCXCL10 | CTGCCCACGTGTTGAGATCA | TGGTCTTAGATTCCGGATTCAGA |
mCD3 | AACACTTTCTGGGGCATCCT | CTGTCTAGAGGGCACGTCAA |
mCD4 | GTTCAGGACAGCGACTTCTG | GAAGGAGAACTCCGCTGACT |
mCD8 | AGTGAAGGGGACCGGATTG | GGACATTTGCAAACACGCTT |
mFoxp3 | CAGAGAGGTATTGAGGGTGGG | GCAGAGTCAGGAGAAGTTGC |
hTBP* | TATAATCCCAAGCGGTTTGC | GCTGGAAAACCCAACTTCTG |
hCYCA* | AGACTGAGTGGTTGGATGGC | TCGAGTTGTCCACAGTCAGC |
hIL-1β | GATGAAGTGCTCCTTCCAGG | GCATCTTCCTCAGCTTGTCC |
hIL-18 | GACTCTTGCGTCAACTTCAAGG | CAGGCTGTCTTTTGTCAACGA |
hIL-6 | GAGGAGACTTGCCTGGTGAA | GCTCTGGCTTGTTCCTCACT |
hICAM-1 | AGCTTCGTGTCCTGTATGGC | ACAGTCACTGATTCCCCGAT |
hVCAM-1 | AATGTTGCCCCCAGAGATACAACCG | GAGCTGCCTGCTCCACAGGA |
hE-selectin | GCACTGTGTGCAAGTTCGC | GGCTTTTGGTAGCTTCCGTC |
hClaudin-1 | TTGACTCCTTGCTGAATCTGAG | TTCTGCACCTCATCGTCTTC |
hClaudin-3 | CATCACGTCGCAGAACATCT | GAGTCGTACACCTTGCACTG |
hClaudin-5 | ACATTGTCGTCCGCGAGTTT | ACTTCTGCGACACGGGCA |
hVE-Cadherin | AAACACCTCACTTCCCCATC | ACCTTGCCCACATATTCTCC |
hPECAM | ATTGCAGTGGTTATCATCGGAGTG | CTCGTTGTTGGAGTTCAGAAGTGG |
hZO-1 | CCCGAAGGAGTTGAGCAGGAAATC | CCACAGGCTTCAGGAACTTGAGG |