Fig. 1

CSF tracer distribution 24 h after photothrombotic stroke. (A) Dye penetration into parenchyma was assessed by quantifying fluorescent parenchymal signal in 15 sequential 50 µm² box regions of interest (ROI) from the pial surface to 750 µm² depth. These ROIs were contained within the region of the coloured boxes shown in B. (B) Representative maximal projection of coronal section from a sham animal indicating the dorsal (blue), lateral (orange), and ventral (green) regions used to quantitate CSF tracer influx. (C) Representative maximal projection of coronal section from a photothrombotic stroke animal. The infarct is outlined in yellow. (D) The mean pixel intensity in the dorsal, lateral, and ventral regions of sham, and stroke ipsilateral and contralateral hemispheres plotted as mean fluorescent pixels/µm from brain surface to give the area under the curve (AUC). **** p < 0.005, *** p < 0.001, **p < 0.01, *p < 0.05. Exposure of the representative images (B and C) was increased for publication.